Size
30 µl
Catalog no.
4800-30-14
Price
130 EUR
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| Value | Description |
|---|---|
| Keep at | -20° C |
| Conjugation | Anti-FITC Antibody |
| More advice | Streptavidin HRP and and TACS Nucleases for positive controls are supplied for immunohistochemistry with HRP an DAB with incorporation of bromodeoxyuridine (BrdU) at the 3’ OH ends of the DNA for apoptosis detection. |
| Assay performance | TACS for in situ detection of apoptosis in a TUNEL test with frozen, paraffin embedded tissues. Enzymatic incorporation of biotinylated nucleotides in fragmented DNA with enzymes and permeabilization reagents, labeling and detection reagents, stop buffers, counterstain and TACS-Nuclease for in situ labeling. |
| Properties | This Trevigen Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Trevigen FITC is highly sensitive to pH extremes. |