Size
96 samples
Catalog no.
4260-096-K
Price
435 EUR
Buy
| Value | Description |
|---|---|
| Keep at | Mixed |
| Assay performance | CometAssay, DNA Damage, PCR and Molecular Biology, Oxidative Damage, PARP research. |
| More advice | For the comet assay procedure GENTAUR also supplies the Comet microscope glass slides. Cellular repair resolves the DNA damage and nucleoids containing specific DNA and the breaks are tested with the comet assay. Performed on Drosophila melanogaster cells or for human DNA damage studies for cancer susceptibility by environmental factors, food habits, sport. A fluorescence microscopy is needed to observe the comet tail where the intensity, length and brightness of the tail indicates the number of DNA lesions and DNA unwinding at neutral pH 7.5. At pH 12.3 the comet assay detects single and double strand breaks, incomplete excision repair sites and cross linking. The DNA amount from the cell nucleus is an indicator of the incurred DNA damage. High throughput is achieved with the 96-Well CometChip the fastest system for high-throughput measurement of DNA damage. Alkali labile sites (ALS) are susceptible of DNA breaching, Miyamae, 1997 and will fast migrate out of the cell from DNA damage, Fairbairn, 1995. The Comet Assay can be applied to both eukaryotic chromosomes and prokaryotic cells. For oncology testing of invasive cancer in the human body the Comet is used by B Vilhar |